|
KMID : 0359320100500030239
|
|
Korean Journal of Veterinary Research
2010 Volume.50 No. 3 p.239 ~ p.246
|
|
|
Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea
|
|
Sung Myung-Suk
Kim Jin-Hyun
Kim Ki-Seuk
|
|
|
Abstract
|
|
|
|
Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern.
|
|
|
KEYWORD
|
|
|
avian pathogenic Escherichia coli (APEC), molecular epidemiological analysis, O serotype, pulsed-field gel electrophoresis (PFGE), restriction endonuclease analysis (REA)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|